Application

Anti-ZKSCAN3 (Zf47), Cat. No. ABC441, is a rabbit polyclonal antibody that detects Zinc finger protein with KRAB and SCAN domains 3 and has been tested for use in Immunocytochemistry and Western Blotting.

Research CategoryApoptosis & Cancer

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZKSCAN3 (Zf47) with KRAB and SCAN domains 3 in HeLa and A431 cell lines.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger protein with KRAB and SCAN domains 3 (UniProt: Q9BRR0; also known as Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306, Zinc finger protein 309, Zinc finger protein 47 homolog, Zf47, Zfp-47) is encoded by the ZKSCAN3 (also known as ZFP47, ZNF306, ZNF309, ZSCAN13) gene (Gene ID: 80317) in human. Zf47 is a member of the krueppel C2H2-type zinc-finger protein family. Two isoforms of Zf47 have been described that are produced by alternative splicing. It contains 7 C2H2 type zinc-fingers. It serves as a transcriptional factor that binds to the consensus sequence 5′-[GT][AG][AGT]GGGG-3′ and acts as a repressor of autophagy. It is shown to specifically repress expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. It is mainly localized in the nucleus, however, under conditions of starvation, it translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function. Overexpression of Zf47 is observed in various tumors, including multiple myeloma, colorectal and prostate cancers.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal half of human Zinc finger protein with KRAB and SCAN domains 3. The immunogen sequence is conserved in both isoforms, 1 and 2.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZKSCAN3 (Zf47) with KRAB and SCAN domains 3 in HeLa cell lysate.

Specificity

This rabbit polyclonal antibody detects Zinc finger protein with KRAB and SCAN domains 3 in human cells. It targets an epitope within 12 amino acids from the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed; 60.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).